ULTRASTRUCTURAL AND CYTOCHEMICAL CHARACTERIZATION OF THE OENOCYTOID IN LARVAE OF DIATRAEA SACCHARALIS (LEPIDOPTERA: PYRALIDAE) PARASITIZED BY THE WASP COTESIA FLAVIPES (HYMENOPTERA: BRACONIDAE)

Resumen

The hemocytes participate in the cellular encapsulation around foreign material implanted into the insect hemocoel. However, the suppression of this reaction by entomopathogens remains largely misunderstood. This study describes the ultrastructural modifications in one type of hemocyte of Diatraea saccharalis larvae, the oenocytoid (OE), along the parasitism by the wasp Cotesia flavipes, using conventional and cytochemical techniques. The OE of parasitized D. saccharalis larvae exhibited areas of cytoplasm rarefaction with intensity proportional to the time of parasitism. There was cell lyse at long-term parasitism, although we did not observe melanization either into the host haemocel or around of parasitoid. The OE of either control or parasitized larvae did not show phenoloxidase activity in the ultrastructural preparations; these absence was related with the morphological modifications caused by the parasitism. The trimetaphosphatase and acid phosphatase activities were not detected in OE of control larvae, but were visualized along the parasitism, reinforcing the role played by the OE in the defense reactions. Key words: Ultrasctructural, oenocytoid, Diatraea saccharalis